Bratanov V, Stoianova-Zaĭkova L, Lilkova N
Vet Med Nauki. 1977;14(1):24-35.
A total of twenty-two experimentally infected pigs, presenting from 250 to 12000 Trichinellae per kilogram body weight, were studied by the skin allergy test, the tube precipitation reaction, and the double gel diffusion technique after Ouchterlony in the course of one year following infection. Use was made of a Trichinella antigen obtained by the Tanner and Gregory's modified method. Unspecific allergy reactions were observed in 2.04% of 104 control pigs, and 2 of the infected ones did not react to the antigen diluted 1:1000. To decrease the cases of unspecific reactions a suggestion was made to extend the antigen from 1:5000 to 1:10000 and to read the result of the reactions not earlier than the 45th min. after injecting the antigen and the physiologic saline. The precipitation reaction proved positive in a comparatively short period--up to the fourth month of infection, and in sporadic cases up to the 7 1/2 month. Unspecific precipitation was observed in 18.7% of a total of 53 control sera. Ouchterlony's diffusion technique was shown to be specific, revealing trichinellosis in the animals from the 15th to the 365th day after the infection had been carried out. It was most markedly expressed from the 30th to the 120th day. It is suggested to use the skin allergy test on farms with a record of swine trichinelloses, all positively reacting pigs being serologically studied after Ouchterlony.
在感染后的一年时间里，对总共22头经实验感染的猪进行了研究，这些猪每千克体重有250至12000条旋毛虫。采用皮肤过敏试验、试管沉淀反应以及奥克特洛尼（Ouchterlony）双凝胶扩散技术。使用的是通过坦纳（Tanner）和格雷戈里（Gregory）改良方法获得的旋毛虫抗原。在104头对照猪中有2.04%出现非特异性过敏反应，2头感染猪对稀释至1:1000的抗原无反应。为减少非特异性反应的情况，建议将抗原稀释度从1:5000提高到1:10000，并在注射抗原和生理盐水后至少45分钟再读取反应结果。沉淀反应在相对较短的时间内呈阳性——感染后直至第四个月，在个别情况下直至7个半月。在总共53份对照血清中有18.7%观察到非特异性沉淀。奥克特洛尼扩散技术显示具有特异性，在感染后第15天至第365天可检测出动物体内的旋毛虫病。在第30天至第120天表现最为明显。建议在有猪旋毛虫病记录的农场采用皮肤过敏试验，所有反应呈阳性的猪在奥克特洛尼试验后进行血清学研究。
