Van De Verg L L, Bendiuk N O, Kotloff K, Marsh M M, Ruckert J L, Puryear J L, Taylor D N, Hartman A B
Department of Enteric Infections, Walter Reed Army Institute of Research, Washington DC 20307-5100, USA.
Vaccine. 1996 Aug;14(11):1062-8. doi: 10.1016/0264-410x(96)00006-0.
To study the cross-reactivity pattern of Shigella flexneri 2a O-antigen antibodies, sera from humans and monkeys challenged with S. flexneri 2a, and from humans and guinea pigs immunized with a recombinant vaccine expressing serotype 2a O-antigen, were tested against a panel of lipopolysaccharide extracted from heterologous S. flexneri. Sera from the two groups of humans, who were volunteers in either a clinical challenge or vaccination study, showed similar patterns: cross-reactivity was more often seen with IgA antibodies, and these were mostly cross-reactive with serotype 2b, which shares the type II antigen, and serotypes 1a, 5a, and Y, which share 4 or 3, 4 group antigen, with 2a. The majority of sera from immunized guinea pigs showed both IgG and IgA cross-reactivity with 1a, 5a, and Y, but not 2b. The majority of sera from challenged monkeys showed cross-reactivity with almost all flexneri serotypes tested, with 1a, 2b, and Y being recognized most often, and the cross-reactive antibodies were more often IgG than IgA. These results show that either immunization or challenge with the 2a serotype induces cross-reactive antibodies which recognize similar subsets of heterologous serotypes, and suggest that it may be possible to design multivalent vaccines against S. flexneri.
为研究福氏志贺菌2a O抗原抗体的交叉反应模式，对用福氏志贺菌2a攻击的人和猴的血清，以及用表达2a血清型O抗原的重组疫苗免疫的人和豚鼠的血清，针对从异源福氏志贺菌提取的一组脂多糖进行了检测。两组人的血清，他们分别是临床攻击或疫苗接种研究的志愿者，显示出相似的模式：IgA抗体更常出现交叉反应，并且这些抗体大多与共享II型抗原的2b血清型，以及与2a共享4或3、4组抗原的1a、5a和Y血清型发生交叉反应。免疫豚鼠的大多数血清显示出与1a、5a和Y的IgG和IgA交叉反应，但与2b没有交叉反应。受攻击猴子的大多数血清与几乎所有测试的福氏志贺菌血清型都有交叉反应，其中1a、2b和Y最常被识别，并且交叉反应抗体中IgG比IgA更常见。这些结果表明，用2a血清型进行免疫或攻击会诱导识别异源血清型相似亚群的交叉反应抗体，并表明有可能设计出针对福氏志贺菌的多价疫苗。
