Hara H, Tanaka A, Kaji A
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
Virus Genes. 1996;12(1):37-46. doi: 10.1007/BF00369999.
The nucleotide sequence of the gene for the envelope protein gp85 (SU) of the Schmidt-Ruppin subgroup A (NY) strain of Rous sarcoma virus [SRA(NY)] was determined, and the deduced amino acid sequence was compared with those of other avian sarcoma-leukosis viruses. Among the five host-range determinant sequences (vr1, vr2, hr1, hr2, and vr3), the host-range determinant sequence hr2 of SRA(NY) showed a significant deviation from the hr2 sequences of other subgroup A viruses namely, RAV-1 and SRA(SF). A phylogenetic analysis of the amino acid sequence of this region indicated that this intra-subgroup diversity was as great as or even greater than the inter-subgroup diversity found among other subgroups of ASLV. Within the hr2 region, we found a short hypervariable segment that differs in length and sequence from hr2 of other subgroup-A viruses. The difference in the hr2 amino acid sequence between SRA(NY) and SRA(SF) is reflected in the predicted protein secondary structure of this region.
测定了劳氏肉瘤病毒施密特-鲁平A亚组（纽约）毒株[SRA(NY)]包膜蛋白gp85（SU）基因的核苷酸序列，并将推导的氨基酸序列与其他禽肉瘤-白血病病毒的序列进行了比较。在五个宿主范围决定序列（vr1、vr2、hr1、hr2和vr3）中，SRA(NY)的宿主范围决定序列hr2与其他A亚组病毒（即RAV-1和SRA(SF)）的hr2序列存在显著差异。对该区域氨基酸序列的系统发育分析表明，这种亚组内的多样性与在禽肉瘤-白血病病毒其他亚组中发现的亚组间多样性一样大甚至更大。在hr2区域内，我们发现了一个短的高变片段，其长度和序列与其他A亚组病毒的hr2不同。SRA(NY)和SRA(SF)之间hr2氨基酸序列的差异反映在该区域预测的蛋白质二级结构中。
