Joliot V, Boroughs K, Lasserre F, Crochet J, Dambrine G, Smith R E, Perbal B
Laboratoire d'Oncologie Virale et Moléculaire, Institut Curie, Centre Universitaire, Orsay, France.
Virology. 1993 Aug;195(2):812-9. doi: 10.1006/viro.1993.1436.
To identify the nucleotide sequences responsible for the tumorigenic specificity of myeloblastosis-associated virus (MAV) we have established the complete nucleotide sequences of three infectious clones inducing either both osteopetrosis and nephroblastoma [MAV2(O)/2 and MAV2(O)p9] or only nephroblastoma [MAV1(N)], and compared their biological properties in the same chicken host strain. The MAV2(O)p9 originally described as a type 2 strain was found to carry a hybrid env gene containing sequences of both the types 1 and 2, and it induced milder and less rapid osteopetrosis than the original MAV2(O) clone when injected into Brown Leghorn chickens. These results, together with sequence comparisons between the MAV strains examined, suggest that subtle changes in the primary structure of the TM env protein's extracellular domain are likely to affect the tumorigenic potential of MAV.
为了鉴定与成髓细胞增多症相关病毒（MAV）致瘤特异性相关的核苷酸序列，我们确定了三个感染性克隆的完整核苷酸序列，这三个克隆分别诱导骨硬化症和肾母细胞瘤[MAV2(O)/2和MAV2(O)p9]或仅诱导肾母细胞瘤[MAV1(N)]，并在同一鸡宿主品系中比较了它们的生物学特性。最初被描述为2型毒株的MAV2(O)p9被发现携带一个包含1型和2型序列的杂交env基因，当将其注射到白来航鸡中时，它诱导的骨硬化症比原始的MAV2(O)克隆更轻且发展更慢。这些结果，连同所检测的MAV毒株之间的序列比较，表明TM env蛋白细胞外结构域一级结构的细微变化可能会影响MAV的致瘤潜力。
