Richardson P, Molloy J, Ravenhall R, Holwill I, Hoare M, Dunnill P
Department of Chemical and Biochemical Engineering, University College London, UK.
J Biotechnol. 1996 Aug 20;49(1-3):111-8. doi: 10.1016/0168-1656(96)01533-7.
Sample clarification is a common operation in biochemical analytical methods for removing interfering or unwanted particulates from an analyte sample. Filtration provides one option for removal of particulates. However, in many cases the loss of soluble protein due to filter adsorption is unacceptable and an alternative must be sought. In this paper a microcentrifuge designed to automatically sample, spin, deliver supernatant to an analyser and wash out solids from the bowl is described. The performance of the system is assessed in terms of its clarification efficiency and the time required to achieve satisfactory clarification. Additionally, the effects of different protein precipitating agents on yeast homogenate samples separated using the microcentrifuge are studied where the system is used to deliver supernatant to a flow injection analyser. The paper demonstrates that the microcentrifuge may be used to separate rapidly such samples on a time scale between 10-60 s depending upon the type and size of sample and be successfully used as a component of an at-line monitoring system.
样品澄清是生化分析方法中的一项常见操作，用于从分析物样品中去除干扰性或不需要的颗粒。过滤是去除颗粒的一种选择。然而，在许多情况下，由于过滤器吸附导致的可溶性蛋白质损失是不可接受的，必须寻找替代方法。本文描述了一种微型离心机，它设计用于自动取样、离心、将上清液输送到分析仪并从转筒中冲洗出固体。该系统的性能根据其澄清效率和达到满意澄清所需的时间进行评估。此外，研究了不同蛋白质沉淀剂对使用微型离心机分离的酵母匀浆样品的影响，该系统用于将上清液输送到流动注射分析仪。本文证明，微型离心机可用于在10 - 60秒的时间范围内快速分离此类样品，具体时间取决于样品的类型和大小，并且可以成功用作在线监测系统的一个组件。
