Dingemans A M, van Ark-Otte J, van der Valk P, Apolinario R M, Scheper R J, Postmus P E, Giaccone G
Department of Medical Oncology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands.
Ann Oncol. 1996 Aug;7(6):625-30. doi: 10.1093/oxfordjournals.annonc.a010681.
The recently discovered LRP protein has been shown to be involved in drug resistance and possibly in detoxification processes.
To study the relation between LRP expression and exposure to cigarette smoke, LRP immunoreactivity was evaluated in 39 paraffin embedded normal lung tissues derived from patients operated on for pneumothorax, and related to amount of pack years smoked. We also studied the LRP protein expression in 36 non-small-cell lung cancer (NSCLC) samples and related the expression to patient characteristics and survival. Furthermore 17 lung tumor samples (10 NSCLC and 7 SCLC) derived from patients treated with chemotherapy were analysed in order to investigate the relation between LRP or MRP expression and the patient's response to chemotherapy.
In the normal lung tissues, LRP intensity levels were not correlated to the amount of pack years smoked, although a trend was seen for higher LRP intensity levels in patients who smoked more than 10 pack years. LRP expression was significantly higher in NSCLC samples than in SCLC samples, and all SCLC samples displayed very low LRP expression. Within NSCLC, squamous cell and adenocarcinomas had higher LRP expression than large cell undifferentiated and mixed tumors. In NSCLC patients LRP expression was not a prognostic factor for survival. At initial analysis LRP expression levels did not predict for the response to chemotherapy. Only 3 out of 17 patients expressed MRP, and all SCLC samples were MRP negative.
Striking different expression levels were seen between NSCLC and SCLC for both LRP and MRP. In a preliminary analysis LRP expression was not predictive for response to chemotherapy in lung cancer patients. In pneumothorax patients LRP levels were not correlated with the amount of pack years smoked.
最近发现的LRP蛋白已被证明与耐药性有关，可能还参与解毒过程。
为研究LRP表达与接触香烟烟雾之间的关系，对39例因气胸接受手术患者的石蜡包埋正常肺组织进行LRP免疫反应性评估，并与吸烟包年数相关联。我们还研究了36例非小细胞肺癌（NSCLC）样本中的LRP蛋白表达，并将其表达与患者特征及生存情况相关联。此外，对17例接受化疗患者的肺肿瘤样本（10例NSCLC和7例SCLC）进行分析，以研究LRP或MRP表达与患者化疗反应之间的关系。
在正常肺组织中，LRP强度水平与吸烟包年数无关，尽管在吸烟超过10包年的患者中观察到LRP强度水平有升高趋势。NSCLC样本中的LRP表达明显高于SCLC样本，所有SCLC样本的LRP表达都非常低。在NSCLC中，鳞状细胞癌和腺癌的LRP表达高于大细胞未分化癌和混合性肿瘤。在NSCLC患者中，LRP表达不是生存的预后因素。初步分析时，LRP表达水平不能预测化疗反应。17例患者中只有3例表达MRP，所有SCLC样本均为MRP阴性。
NSCLC和SCLC在LRP和MRP的表达水平上存在显著差异。在初步分析中，LRP表达不能预测肺癌患者的化疗反应。在气胸患者中，LRP水平与吸烟包年数无关。
