Storck J, Del Razek A, Zimmermann E R
University Münster, Institute of Vegetative Physiology, Germany.
Biomaterials. 1996 Sep;17(18):1791-4. doi: 10.1016/0142-9612(95)00360-6.
In this work, human umbilical vein endothelial cells were cultured on common polystyrene cell culture plates, referred to as control plates, as well as on soft polyvinyl chloride plastics (PVC). Growth of human umbilical endothelial cells (HUVEC) on PVC coated with gelatin, collagen A and heparin plasma was significantly less than that on the control plates coated with the same substance or fibronectin. Cells cultured on PVC produced up to four times as much tissue plasminogen activator than control cells. With reference to plasminogen activator inhibitor 1 (PAI-1), more PAI-1 was released from cells grown on PVC than from those on the control plates coated with gelatin and collagen A. After endotoxin stimulation, the PAI-1 release of HUVEC cultured on PVC was significantly higher than that of control cells with the exception of cells grown on the fibronectin-coated PVC that showed no difference. It is concluded that the type of plastic and coat used to culture HUVEC play a definite role in their growth and function.
在本研究中，人脐静脉内皮细胞在普通聚苯乙烯细胞培养板（称为对照板）以及软质聚氯乙烯塑料（PVC）上进行培养。人脐静脉内皮细胞（HUVEC）在涂有明胶、胶原蛋白A和肝素血浆的PVC上的生长明显少于在涂有相同物质或纤连蛋白的对照板上的生长。在PVC上培养的细胞产生的组织纤溶酶原激活物比对照细胞多四倍。关于纤溶酶原激活物抑制剂1（PAI-1），在PVC上生长的细胞释放的PAI-1比在涂有明胶和胶原蛋白A的对照板上生长的细胞释放的更多。在内毒素刺激后，除了在纤连蛋白包被的PVC上生长的细胞无差异外，在PVC上培养的HUVEC的PAI-1释放明显高于对照细胞。得出结论，用于培养HUVEC的塑料类型和包被物对其生长和功能起着一定作用。
