Thin-layer chromatographic determination of catecholamines in rat plasma.

作者信息

Alemany G, Akaârir M, Rosselló C, Gamundi A

机构信息

Dept. de Biología Fonamental i Ciencies de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.

出版信息

Biomed Chromatogr. 1996 Sep-Oct;10(5):225-7. doi: 10.1002/(SICI)1099-0801(199609)10:5<225::AID-BMC587>3.0.CO;2-T.

A TLC method for the quantitative determination of epinephrine, norepinephrine and dopamine in rat plasma is described. After deprotenization, catecholamines were adsorbed on acid-alumina and acetylated. The acetyl derivatives were extracted using a C18 minicolumn, resolved on HPTLC plates and quantitated by fluorescence densitometry at 415 nm, using isoprenaline as internal standard.