Day M J
Department of Pathology and Microbiology, University of Bristol, Langford.
Res Vet Sci. 1996 Sep;61(2):129-35. doi: 10.1016/s0034-5288(96)90087-9.
A panel of monoclonal antibodies (mAbs) reactive with the four canine IgG subclasses (IgG1, IgG2, IgG3 and IgG4) was used to investigate the activity of IgG subclasses in autoimmune responses to erythrocytes, nuclear antigens and thyroglobulin in dogs with autoimmune disease. mAbs specific for canine IgG1 and IgG4 were able to agglutinate normal dog erythrocytes coated with purified canine IgG containing each of the four subclasses. IgG1 autoantibodies were demonstrated on the surface of erythrocytes from seven dogs with Coombs'-positive autoimmune haemolytic anaemia and IgG4 autoantibodies were also present in two of these cases. Serum antinuclear antibody was shown in nine dogs to be predominantly of IgG1, IgG3 and IgG4 subclasses with only one of the dogs also producing an IgG2 antinuclear antibody. The IgG subclass profile of antinuclear antibodies differed between dogs with polyarthritis and dogs with other systemic disease. In six dogs with hypothyroidism, thyroglobulin autoantibodies of subclasses, IgG1, IgG2 and IgG4 predominated, but one dog also produced significant levels of IgG3 thyroglobulin autoantibodies. The mAbs are widely applicable to the study of the pathogenesis of canine autoimmune disease.
一组与四种犬IgG亚类（IgG1、IgG2、IgG3和IgG4）反应的单克隆抗体（mAb）被用于研究自身免疫性疾病犬对红细胞、核抗原和甲状腺球蛋白的自身免疫反应中IgG亚类的活性。对犬IgG1和IgG4特异的mAb能够凝集包被有含四种亚类的纯化犬IgG的正常犬红细胞。在7只库姆斯试验阳性的自身免疫性溶血性贫血犬的红细胞表面证实有IgG1自身抗体，其中2只犬还存在IgG4自身抗体。9只犬的血清抗核抗体显示主要为IgG1、IgG3和IgG4亚类，只有1只犬还产生IgG2抗核抗体。多关节炎犬和其他全身性疾病犬的抗核抗体的IgG亚类谱不同。在6只甲状腺功能减退犬中，IgG1、IgG2和IgG4亚类的甲状腺球蛋白自身抗体占主导，但1只犬也产生了高水平的IgG3甲状腺球蛋白自身抗体。这些mAb广泛适用于犬自身免疫性疾病发病机制的研究。
