Dick J E
Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
Semin Immunol. 1996 Aug;8(4):197-206. doi: 10.1006/smim.1996.0025.
Understanding the processes that regulate the developmental program of normal stem cells and those that initiate proliferative diseases such as leukemia remains one of the major challenges in biology. Progress to address these major questions in the human hematopoietic system have been hampered, until recently, by the lack of in-vivo assays for normal and leukemic stem cells. The recent development of methods to transplant normal and leukemic human hematopoietic cells into immune-deficient mice provides an important approach to identify, characterize and purify stem cells. This review will focus on the development of assays for normal and leukemic human stem cells and on the new insights these assays are beginning to provide on the organization of the human stem cell hierarchy and mechanisms of leukemogenesis.
了解调控正常干细胞发育程序的过程以及引发白血病等增殖性疾病的过程，仍然是生物学领域的主要挑战之一。直到最近，由于缺乏针对正常和白血病干细胞的体内检测方法，在人类造血系统中解决这些重大问题的进展一直受到阻碍。最近将正常和白血病人类造血细胞移植到免疫缺陷小鼠体内的方法的发展，为鉴定、表征和纯化干细胞提供了一种重要途径。本综述将聚焦于针对正常和白血病人类干细胞检测方法的发展，以及这些检测方法开始为人类干细胞层级组织和白血病发生机制提供的新见解。
