Analysis of connective tissue macromolecular components in Ishibashi rat skin: role of collagen and elastin in cutaneous aging.

The Ishibashi (IS) rat, established from cross-breeding between Wistar and wild rats, has a unique skin appearance, with wrinklings and furrows indicative of skin aging appearing at the age of 12 weeks. To understand the underlying mechanism of the formation of wrinkles, macromolecular components of connective tissue, collagen and elastin, in the young (5-6-week-old) and the aged (23-30-week-old) IS rat skins were biochemically analyzed. Hydroxyproline and isodesmosine contents in the aged IS rats were reduced 22% (P < 0.05) and 37% (P < 0.05) compared to the young rats, whereas no significant differences in the contents of both macromolecules in control Sprague-Dawley (SD) rats were seen. The relative content of type III collagen was unaltered between the young and aged skins of both IS and SD rats. A relative decrease in the intact elastin molecule (65 kDa) and a relative increase in the elastin fragments with lower molecular weights were observed in the aged IS rat skin by immunoblotting method. These results indicate that the reduction in collagen and elastin contents and increased degradation of elastin molecules in the aged IS rat skin could be related to the formation of wrinkles. Thus, the IS rat may provide a useful model for the study of skin aging.