Friel J K, Mercer C, Andrews W L, Simmons B R, Jackson S E, Longerich H P
Department of Biochemistry, Memorial University, St. John's, Newfoundland, Canada.
Biol Trace Elem Res. 1996 Aug;54(2):135-42. doi: 10.1007/BF02786260.
Contamination in a trace element laboratory can come from a variety of sources, including laboratory gloves. Therefore, vinyl and latex gloves were obtained from as many manufacturers as would supply gloves. These gloves were either prepared for acid-washing and subsequent soaking in an acid solution, or immersed in an acid solution for a duration of either 1 min or 1 h. Incubation washes were analyzed for a variety of trace elements by flame atomic absorption spectroscopy (AAS) or inductively coupled mass spectrometry (ICP-MS). Results indicated that only three brands of vinyl gloves were acceptable for use in a trace element laboratory, whereas others had contamination of different elements. Latex gloves contained such high levels of biologically important elements that they were not considered suitable for routine trace element work. Vinyl gloves of choice should be routinely acid-washed before use in a trace element laboratory.
痕量元素实验室中的污染可能来自多种来源,包括实验室手套。因此,从尽可能多能提供手套的制造商那里获取了乙烯基手套和乳胶手套。这些手套要么准备进行酸洗并随后浸泡在酸溶液中,要么在酸溶液中浸泡1分钟或1小时。通过火焰原子吸收光谱法(AAS)或电感耦合质谱法(ICP-MS)对孵育洗涤液中的多种痕量元素进行了分析。结果表明,只有三个品牌的乙烯基手套可用于痕量元素实验室,而其他品牌则含有不同元素的污染。乳胶手套中含有如此高水平的生物重要元素,以至于它们不被认为适合进行常规痕量元素工作。在痕量元素实验室使用之前,应常规对所选的乙烯基手套进行酸洗。