Xiao Y M, Diao Y C, So K F
Laboratory of Visual Information Processing, Academia Sinica, Beijing, China.
Brain Behav Evol. 1996;48(4):221-30. doi: 10.1159/000113200.
The distribution of the enzyme nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase, which is identical to nitric oxide synthase (NOS), was examined in cortical area 17 and the 17/18a border region in the brain of the golden hamster. The activity of the enzyme was present as a network of processes and in special populations of neurons in the visual cortex. The dense enzyme-positive network exhibited numerous varicosities distributed throughout the cortex. The prominent orientation of the processes in layer I and the white matter are parallel to the surface of the brain, but those in layers II-IV are perpendicular to the surface of the brain. However, the processes in layers V and VI seem to run randomly. The NADPH diaphorase-positive cells could be divided into two classes: heavily stained neurons and lightly stained neurons. For the lightly stained NADPH diaphorase-positive neurons, only the cell bodies could be observed, whereas for the heavily stained neurons, the cell bodies and their varicosity-carrying dendrites and, occasionally, the smooth, thin and weakly stained axons were visible. The heavily stained neurons were morphologically diverse, but no pyramidal or spiny neurons were found. Multipolar and bipolar neurons were located throughout the depth of the cortex, including the white matter, more frequently in layers V and VI. Occasionally, monopolar neurons were found in layer VI. Callosal projecting neurons in the visual cortex were labeled retrogradely with the use of FluoSpheres applied at the opposite visual cortex, but these neurons did not co-localize with the NADPH diaphorase-positive neurons, suggesting that the callosal projecting neurons and NADPH diaphorase-positive neurons belong to two populations of cells in the visual cortex.
在金黄地鼠大脑的17区皮质以及17/18a边界区域，研究了与一氧化氮合酶（NOS）相同的烟酰胺腺嘌呤二核苷酸磷酸（NADPH）黄递酶的分布情况。该酶的活性以神经突起网络的形式存在于视皮质的特定神经元群体中。密集的酶阳性网络在整个皮质中呈现出众多的膨体。I层和白质中的神经突起的突出方向与脑表面平行，但II - IV层中的神经突起与脑表面垂直。然而，V层和VI层中的神经突起似乎是随机分布的。NADPH黄递酶阳性细胞可分为两类：深染神经元和浅染神经元。对于浅染的NADPH黄递酶阳性神经元，只能观察到细胞体，而对于深染神经元，细胞体及其带有膨体的树突，偶尔还有光滑、细且染色浅的轴突都清晰可见。深染神经元在形态上多种多样，但未发现锥体神经元或棘状神经元。多极和双极神经元分布于皮质各层的深度，包括白质，在V层和VI层更为常见。偶尔在VI层发现单极神经元。通过在对侧视皮质应用荧光球逆行标记视皮质中的胼胝体投射神经元，但这些神经元与NADPH黄递酶阳性神经元不共定位，这表明胼胝体投射神经元和NADPH黄递酶阳性神经元属于视皮质中的两个细胞群体。
