Herrmann-Rinke C, Eissele R, Arnold R, Göke B
Department of Internal Medicine, Philipps University of Marburg, Germany.
Digestion. 1996;57(5):349-55. doi: 10.1159/000201356.
The colon contains large numbers of endocrine cells. Insight into their physiological function is limited. This is due to the fact that no sufficient model of isolated endocrine colon cells is available. In the present study we introduce an isolated vascularly perfused colon model for in vitro studies. This model offers the advantage that it keeps the endocrine cells in their physiological orientation and environment. The gut mucosa is highly sensitive to ischemia. Therefore, a careful validation of its viability is crucial in gut organ preparations. This study demonstrates that, by utilizing an oxygenated vascular medium supplemented with 25% washed bovine erythrocytes, a perfusion of the colon is achieved for at least 1 h without obvious tissue injuries. During this time parameters such as perfusion pressure, venous lactate dehydrogenase release, glucose consumption, lactate output, oxygen consumption, perfusate loss by the preparation and morphology were analyzed. Dependent on stimulation, the endocrine L cells of the colon released glucagon-like peptide-I upon arterial perfusion of methacholine or gastrin-releasing peptide. In conclusion, a model for the isolated perfusion of the colon is introduced which is suitable for studies of endocrine colon cells.
结肠中含有大量内分泌细胞。对其生理功能的了解有限。这是因为目前尚无足够的分离结肠内分泌细胞模型。在本研究中，我们引入了一种用于体外研究的离体血管灌注结肠模型。该模型的优点是能使内分泌细胞保持其生理取向和环境。肠黏膜对缺血高度敏感。因此，在肠道器官制备中仔细验证其活力至关重要。本研究表明，通过使用补充有25%洗涤过的牛红细胞的含氧血管介质，可实现结肠灌注至少1小时而无明显组织损伤。在此期间，分析了诸如灌注压力、静脉乳酸脱氢酶释放、葡萄糖消耗、乳酸输出、氧消耗、制剂的灌注液损失和形态等参数。根据刺激情况，在动脉灌注乙酰甲胆碱或胃泌素释放肽时，结肠的内分泌L细胞会释放胰高血糖素样肽-1。总之，我们引入了一种适合研究结肠内分泌细胞的离体结肠灌注模型。
