Hansen C P, Stadil F, Rehfeld J F
Department of Gastrointestinal Surgery, Rigshospitalet, University of Copenhagen, Denmark.
Digestion. 1996;57(5):356-61. doi: 10.1159/000201357.
The elimination of endogenous carboxyamidated and glycine-extended gastrins in liver and gut was studied before and after feeding in 14 anesthetized pigs. Before the meal, liver and gut extractions were nonsignificant. After feeding, the release rate of amidated gastrin increased from 7.5 +/- 2.6 to 21.9 +/- 5.3 pmol/min (p < 0.02), and the liver extracted significant amounts of amidated gastrin, while the intestinal extraction remained nonsignificant. The postprandial hepatic extraction ratio increased from 0.09 +/- 0.04 to 0.18 +/- 0.04 (p < 0.01). Before feeding, the hepatic and extrahepatic clearance rates were 80.0 +/- 38.7 and 232.3 +/- 77.7 ml/min. Clearance rates after feeding were 144.8 +/- 29.5 (p < 0.01) and 326.4 +/- 75.3 ml/min (NS), respectively. Portal plasma displayed a small postprandial increase in the concentration of glycine-extended gastrin, but extraction over the liver and gut remained nonsignificant. Gel chromatography of portal plasma showed that the fraction of postprandial amidated gastrin corresponded to gastrin-17. The concentration of glycine-extended gastrin was too low for chromatographic analysis. We conclude that endogenous amidated gastrin is eliminated in the liver after feeding in pigs.
在14头麻醉猪进食前后，研究了肝脏和肠道对内源性羧基酰胺化胃泌素和甘氨酸延伸胃泌素的清除情况。进食前，肝脏和肠道的提取量不显著。进食后，酰胺化胃泌素的释放速率从7.5±2.6皮摩尔/分钟增加到21.9±5.3皮摩尔/分钟（p<0.02），肝脏提取了大量的酰胺化胃泌素，而肠道提取量仍不显著。餐后肝脏提取率从0.09±0.04增加到0.18±0.04（p<0.01）。进食前，肝脏和肝外清除率分别为80.0±38.7和232.3±77.7毫升/分钟。进食后的清除率分别为144.8±29.5（p<0.01）和326.4±75.3毫升/分钟（无显著差异）。门静脉血浆中甘氨酸延伸胃泌素的浓度在餐后略有增加，但肝脏和肠道的提取量仍不显著。门静脉血浆的凝胶色谱分析表明，餐后酰胺化胃泌素的组分对应于胃泌素-17。甘氨酸延伸胃泌素的浓度过低，无法进行色谱分析。我们得出结论，猪进食后肝脏可清除内源性酰胺化胃泌素。
