Paetau A, Virtanen I, Stenman S, Kurki P, Linder E, Vaheri A, Westermark B, Dahl D, Haltia M
Acta Neuropathol. 1979 Jun 15;47(1):71-4. doi: 10.1007/BF00698276.
Cultured human glioma cells were studied by double indirect immunofluorescence technique using antisera against intermediate filaments and glial fibrillary acidic protein. With both antisera cytoplasmic fibrillar fluorescence was seen. Perinuclear bundles of intermediate-sized filaments, induced by vinblastine treatment, were strongly stained with both antisera. The degree of codistribution of the two types of antigenic determinants varied considerably from cell to cell. The results suggest that two types of filament-related antigenic determinants can be present in the same cell, and also that glial fibrillary acidic protein-related filaments may possess functional similarities to the intermediate filaments found in other cells. Glial fibrillary acidic protein remains as a useful and specific antigenic marker for the study of glial cells in vitro.
利用抗中间丝和胶质纤维酸性蛋白的抗血清，通过双重间接免疫荧光技术对培养的人胶质瘤细胞进行了研究。两种抗血清均可见细胞质纤维状荧光。经长春碱处理诱导产生的中等大小中间丝的核周束，用两种抗血清染色均呈强阳性。两种抗原决定簇的共分布程度在不同细胞间差异很大。结果表明，两种类型的丝状相关抗原决定簇可存在于同一细胞中，并且与胶质纤维酸性蛋白相关的丝可能与其他细胞中的中间丝具有功能相似性。胶质纤维酸性蛋白仍然是体外研究胶质细胞的一种有用且特异的抗原标志物。
