Griffith J S, Turner G D
AgrEvo Environmental Health Ltd, Berkhamsted, Herts., U.K.
Med Vet Entomol. 1996 Jul;10(3):265-8. doi: 10.1111/j.1365-2915.1996.tb00741.x.
The tropical house mosquito Culex quinquefasciatus was cultured by feeding the females on an artificial diet, not on live animals or whole blood. This anautogenous strain has been maintained for more than fifty generations. The blood replacement diet for female mosquitoes, designed to simulate the tonicity and density of host blood, was based on ovalbumin, soya infant formula, globulins and adenosine triphosphate. Female adults of Cx quinquefasciatus were fed the artificial blood formula from "Parafilm' wax membrane-covered beakers. The diet was heated by radiant heat from a chamber containing an exothermic chemical reaction. This maintained the diet at a temperature of 33-37 degrees C for a period of up to 6 h, sufficient time to enable all the female mosquitoes to imbibe to satiation. After six generations on the artificial diet, female fecundity stabilized to a satisfactory level: the number of eggs per gonotrophic cycle averaged c. 85% of the "control' strain fed on whole blood from live anaesthetized guineapigs, i.e. 156 eggs per female from nine feeds on the artificial diet compared with 183 from six feeds of whole blood. Adult weight of Cx quinquefasciatus females was not significantly different, from generation 6 onwards, for strains fed on artificial diet or whole blood. Sex ratio and the rate of egg viability were also equivalent for the two strains.
热带家蚊致倦库蚊通过给雌蚊喂食人工饲料而非活体动物或全血来进行培养。这种非嗜血性品系已经维持了五十多代。用于雌蚊的血液替代饲料旨在模拟宿主血液的张力和密度，其以卵清蛋白、大豆婴儿配方奶粉、球蛋白和三磷酸腺苷为基础。致倦库蚊的成年雌蚊从覆盖有“Parafilm”蜡膜的烧杯中摄取人工血液配方饲料。饲料通过一个包含放热化学反应的腔室发出的辐射热进行加热。这可使饲料在33 - 37摄氏度的温度下保持长达6小时，这段时间足以让所有雌蚊饱食。在食用人工饲料六代后，雌蚊的繁殖力稳定在一个令人满意的水平：每个生殖营养周期的卵数平均约为以麻醉的活体豚鼠全血为食的“对照”品系的85%，即食用人工饲料九次后每只雌蚊产156枚卵，而食用全血六次后为183枚卵。从第六代起，食用人工饲料或全血的致倦库蚊雌蚊的成虫体重没有显著差异。两种品系的性别比例和卵的活力率也相当。
