Pawlowski W P, Somers D A
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul 55108, USA.
Mol Biotechnol. 1996 Aug;6(1):17-30. doi: 10.1007/BF02762320.
Microprojectile bombardment to deliver DNA into plant cells represents a major breakthrough in the development of plant transformation technologies and accordingly has resulted in transformation of numerous species considered recalcitrant to Agrobacterium- or protoplast-mediated transformation methods. This article attempts to review the current understanding of the molecular and genetic behavior of transgenes introduced by microprojectile bombardment. The characteristic features of the transgene integration pattern resulting from DNA delivery via microprojectile bombardment include integration of the full length transgene as well as rearranged copies of the introduced DNA. Copy number of both the transgene and rearranged fragments is often highly variable. Most frequently the multiple transgene copies and rearranged fragments are inherited as a single locus. However, a variable proportion of transgenic events produced by microprojectile bombardment exhibit Mendelian ratios for monogenic and digenic segregation vs events exhibiting segregation distortion. The potential mechanisms underlying these observations are discussed.
通过微粒轰击将DNA导入植物细胞是植物转化技术发展中的一项重大突破，因此已实现了许多被认为难以用农杆菌介导或原生质体介导转化方法转化的物种的转化。本文试图综述目前对通过微粒轰击导入的转基因的分子和遗传行为的理解。通过微粒轰击进行DNA传递所产生的转基因整合模式的特征包括全长转基因的整合以及导入DNA的重排拷贝。转基因和重排片段的拷贝数通常高度可变。最常见的是，多个转基因拷贝和重排片段作为一个单一位点遗传。然而，与表现出分离畸变的事件相比，微粒轰击产生的可变比例的转基因事件表现出单基因和双基因分离的孟德尔比率。文中讨论了这些观察结果背后的潜在机制。
