Durrant I, Stone T
Amersham International, Bucks, UK.
Mol Biotechnol. 1996 Aug;6(1):65-7. doi: 10.1007/BF02762324.
The direct labeling of nucleic acid probes with horseradish peroxidase (HRP) may be used in many membrane hybridization applications, including Southern blots, Northern blots, colony and plaque screening, PCR products detection/identification. This article describes the preparation method, which involves the labeling of a single-stranded nucleic acid probe with a positively charged HRP-parabenzoquinone-polyethyleneimine complex (labeling reagent). The associated hybridization and posthybridization protocols are relatively simple, which makes probes labeled directly with HRP particularly suitable for large scale screening, where tens or hundreds of blots are processed weekly.
用辣根过氧化物酶（HRP）直接标记核酸探针可用于许多膜杂交应用，包括Southern印迹、Northern印迹、菌落和噬菌斑筛选、PCR产物检测/鉴定。本文介绍了制备方法，该方法涉及用带正电荷的HRP-对苯醌-聚乙烯亚胺复合物（标记试剂）标记单链核酸探针。相关的杂交和杂交后方案相对简单，这使得直接用HRP标记的探针特别适用于大规模筛选，即每周处理数十或数百个印迹。
