Renz M, Kurz C
Nucleic Acids Res. 1984 Apr 25;12(8):3435-44. doi: 10.1093/nar/12.8.3435.
A general method has been developed which allows crosslinks to be produced between proteins and single-stranded DNA. Such single-stranded DNA protein complexes have been tested for blot hybridization using two colorimetrically detectable enzymes, namely peroxidase and alkaline phosphatase, as the protein moiety of the probe. After hybridization and incubation with a substrate solution sequences complementary to the probe can be visualized directly without the need of tedious cytochemical sandwich methods. This procedure will detect target sequences, a few kilobases long, in the 1- to 5-pg range.
已开发出一种通用方法，可在蛋白质与单链DNA之间产生交联。已使用两种比色可检测酶，即过氧化物酶和碱性磷酸酶，作为探针的蛋白质部分，对这种单链DNA - 蛋白质复合物进行印迹杂交测试。杂交并与底物溶液孵育后，与探针互补的序列可直接可视化，无需繁琐的细胞化学夹心方法。该程序可检测1至5皮克范围内几千碱基长的靶序列。
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