硬脑膜和周围神经的激光组织焊接：一项扫描电子显微镜研究。
Laser tissue welding of dura mater and peripheral nerves: a scanning electron microscopy study.
作者信息
Menovsky T, Beek J F, van Gemert M J
机构信息
Laser Center, Academic Medical Center, Amsterdam, The Netherlands.
出版信息
Lasers Surg Med. 1996;19(2):152-8. doi: 10.1002/(SICI)1096-9101(1996)19:2<152::AID-LSM5>3.0.CO;2-R.
BACKGROUND AND OBJECTIVE
In order to elucidate the mechanism of tissue welding, scanning electron microscopy (SEM) was used to investigate the ultrastructural changes on the surface of dura mater and peripheral nerves after CO2 laser welding.
STUDY DESIGN/MATERIALS AND METHODS: The dura mater and the epineurium of the nerves was welded with a CO2 laser at 100 mW with pulses of 1.0 s (spot size 320 microns), both with and without additional use of a protein solder (egg white). The specimens were immediately examined using SEM.
RESULTS
The laser tissue bonding mechanism is collagen-to-collagen attachment. After laser irradiation, the collagen fibrils are swollen, densely packed, and fused together. When a protein solder is used, the coagulated solder forms a solid bridge between the tissue edges, which is melted on and between the collagen fibrils.
CONCLUSION
Laser welds in dura mater and peripheral nerves are the result of collagen-to-collagen bonding. In solder-assisted laser welds, the tissue connection is made by an internal and an external matrix of coagulated solder.
背景与目的
为阐明组织焊接的机制，采用扫描电子显微镜（SEM）研究二氧化碳激光焊接后硬脑膜和周围神经表面的超微结构变化。
研究设计/材料与方法：使用二氧化碳激光以100毫瓦、1.0秒脉冲（光斑尺寸320微米）对硬脑膜和神经外膜进行焊接，分别在使用和不使用蛋白焊料（蛋清）的情况下进行。标本立即用扫描电子显微镜检查。
结果
激光组织黏合机制是胶原蛋白与胶原蛋白的附着。激光照射后，胶原纤维肿胀、紧密排列并融合在一起。使用蛋白焊料时，凝固的焊料在组织边缘形成坚固的桥接，该桥接在胶原纤维上和之间熔化。
结论
硬脑膜和周围神经的激光焊接是胶原蛋白与胶原蛋白结合的结果。在焊料辅助激光焊接中，组织连接是由凝固焊料的内部和外部基质形成的。
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