Wilson A W, Neill J C, Costall B
School of Pharmacy, University of Bradford, West Yorkshire, UK.
Alcohol. 1996 Sep-Oct;13(5):407-13. doi: 10.1016/0741-8329(95)02110-8.
Agents affecting serotonergic (5-hydroxytryptamine, 5-HT) function influence ethanol consumption in rats and primates. In the present study female Sprague-Dawley rats were trained to orally self-administer 8% ethanol (v/v) in a large operant chamber in a 60-min test period by a prandial drinking technique. The number of response, ethanol reinforcers (dipper deliveries), and ethanol consumption (g/kg) were measured following administration of the 5-HT1 A agonist 8-OH-DPAT (0.001 1.0 mg/kg, ip) 30 min prior to testing. Locomotor activity (LMA) was also measured to assess activity changes induced by 8-OH-DPAT. 8-OH-DPAT selectively reduced ethanol ingestion from 17.1 +/- 3.2 dipper deliveries under vehicle conditions to 6.6 +/- 3 at a dose of 0.1 mg/kg. Higher doses of 8-OH-DPAT (0.5 and 1.0 mg/kg) significantly reduced both ethanol ingestion and LMA. Lower doses of 0.001-0.01 mg/kg of 8-OH-DPAT were without effect on ethanol intake and maintained behavior. These results demonstrate that, under the present experimental conditions, the 5-HT1A receptor agonist 8-OH-DPAT reduced ethanol self-administration in the rat, and support a role for 5-HT1A receptors in the mediation of ethanol reinforcement.
影响血清素能（5-羟色胺，5-HT）功能的药物会影响大鼠和灵长类动物的乙醇摄入量。在本研究中，通过餐后饮水技术，训练雌性Sprague-Dawley大鼠在一个大型操作箱中在60分钟的测试期内口服自行摄入8%乙醇（v/v）。在测试前30分钟腹腔注射5-HT1A激动剂8-OH-DPAT（0.001 - 1.0毫克/千克）后，测量反应次数、乙醇强化物（水瓢递送次数）和乙醇摄入量（克/千克）。还测量了运动活性（LMA）以评估8-OH-DPAT引起的活性变化。8-OH-DPAT以0.1毫克/千克的剂量选择性地将乙醇摄入量从载体条件下的17.1±3.2次水瓢递送减少到6.6±3次。更高剂量的8-OH-DPAT（0.5和1.0毫克/千克）显著降低了乙醇摄入量和LMA。较低剂量的0.001 - 0.01毫克/千克的8-OH-DPAT对乙醇摄入量和维持行为没有影响。这些结果表明，在当前实验条件下，5-HT1A受体激动剂8-OH-DPAT减少了大鼠的乙醇自我给药，并支持5-HT1A受体在乙醇强化介导中的作用。
