Rice-Evans C, Leake D, Bruckdorfer K R, Diplock A T
Division of Biochemistry and Molecular Biology, United Medical School of Guy's Hospital, London.
Free Radic Res. 1996 Oct;25(4):285-311. doi: 10.3109/10715769609149053.
The purpose of this review is to bring together the different approaches for studying the oxidation of low density lipoproteins and try to identify some critical factors which will permit greater comparability between laboratories. These issues are discussed both in terms of the variety of exogenous mediators of oxidation applied (transition metal ions, haem proteins, azo initiators, peroxynitrite, cells etc.) and their raisons d'être, as well as the methodologies (formation of conjugated dienes, hydroperoxides, decomposition products of lipid peroxidation, altered surface charge, macrophage uptake) applicable to the different stages of the oxidation and the factors underlying their accurate execution and interpretation.
本综述的目的是汇集研究低密度脂蛋白氧化的不同方法，并试图确定一些关键因素，以使各实验室之间具有更高的可比性。将从所应用的各种外源性氧化介质（过渡金属离子、血红素蛋白、偶氮引发剂、过氧亚硝酸盐、细胞等）及其存在的理由，以及适用于氧化不同阶段的方法（共轭二烯、氢过氧化物的形成、脂质过氧化的分解产物、表面电荷改变、巨噬细胞摄取）及其准确实施和解释的潜在因素等方面来讨论这些问题。
