Hilton I, Dearman R J, Fielding I, Basketter D A, Kimber I
Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, UK.
J Appl Toxicol. 1996 Sep-Oct;16(5):459-64. doi: 10.1002/(SICI)1099-1263(199609)16:5<459::AID-JAT366>3.0.CO;2-H.
The sensitizing properties of the fragrances eugenol and isoeugenol have been investigated experimentally. The potential of these materials to induce sensitization of the respiratory tract was examined using the mouse IgE test, a novel but as yet unvalidated method for the predictive identification of chemical respiratory allergens. Comparisons were made with the activity of eugenol and isoeugenol in two predictive tests for contact sensitization potential: the murine local lymph node assay and the guinea pig maximization test. Both chemicals elicited positive responses in these tests, isoeugenol exhibiting a greater potential for contact sensitization than eugenol. Isoeugenol was negative at all concentrations examined in the mouse IgE test. In the same assay, exposure to eugenol was associated with a statistically significant increase in serum IgE concentrations when initial application concentrations of 2.5% were used. However, at higher test concentrations eugenol was negative in the mouse IgE test. It is concluded that neither eugenol nor isoeugenol have a significant potential to cause sensitization of the respiratory tract, a conclusion that is apparently consistent with the lack of evidence for occupational respiratory allergy associated with exposure to these chemicals. The evidence for isoeugenol lacking respiratory sensitization activity is particularly strong and it is proposed that this chemical may be of value as a negative control in the development and validation of new predictive test methods for the identification of chemical respiratory allergens.
已通过实验研究了丁香酚和异丁香酚香料的致敏特性。使用小鼠IgE试验检测了这些物质诱发呼吸道致敏的可能性，该试验是一种新型但尚未经过验证的预测性鉴定化学性呼吸道过敏原的方法。将丁香酚和异丁香酚在两种接触致敏可能性预测试验中的活性进行了比较：小鼠局部淋巴结试验和豚鼠最大化试验。两种化学物质在这些试验中均引发了阳性反应，异丁香酚表现出比丁香酚更大的接触致敏潜力。异丁香酚在小鼠IgE试验中所有检测浓度下均呈阴性。在同一试验中，当使用2.5%的初始应用浓度时，接触丁香酚与血清IgE浓度的统计学显著增加有关。然而，在更高的试验浓度下，丁香酚在小鼠IgE试验中呈阴性。结论是丁香酚和异丁香酚均没有显著的引起呼吸道致敏的潜力，这一结论显然与缺乏与接触这些化学物质相关的职业性呼吸道过敏的证据一致。异丁香酚缺乏呼吸道致敏活性的证据尤为有力，有人提出这种化学物质在开发和验证用于鉴定化学性呼吸道过敏原的新预测试验方法中可能作为阴性对照具有价值。
