基于图谱的AFLP指纹技术用于水稻基因组扫描

Towards rice genome scanning by map-based AFLP fingerprinting.

作者信息

Zhu J H, Stephenson P, Laurie D A, Li W, Tang D, Gale M D

机构信息

John Innes Centre, Norwich Research Park, Colney, UK.

出版信息

Mol Gen Genet. 1999 Feb;261(1):184-95. doi: 10.1007/s004380050956.

DOI:10.1007/s004380050956

PMID:10071225

Abstract

Map-based DNA fingerprinting with AFLP markers provides a fast method for scanning the rice genome. Three hundred AFLP markers identified with ten primer combinations were mapped in two rice populations. The genetic maps were aligned and almost full coverage of the rice genome was obtained. The transferability of AFLP markers between indica x japonica and indica x indica crosses was tested. The chromosomes were divided into DNA Fingerprint Linkage Blocks (DFLBs) defined by specific AFLP markers. Using these blocks, the degree of similarity or divergence within specific chromosome regions was calculated for nine varieties. Applications of map-based fingerprinting for biodiversity studies and maker-assisted selection are discussed.

摘要

基于扩增片段长度多态性（AFLP）标记的图谱DNA指纹技术为扫描水稻基因组提供了一种快速方法。利用十种引物组合鉴定出的300个AFLP标记被定位到两个水稻群体中。对这些遗传图谱进行了比对，获得了几乎覆盖整个水稻基因组的结果。测试了AFLP标记在籼稻×粳稻和籼稻×籼稻杂交组合之间的可转移性。染色体被划分为由特定AFLP标记定义的DNA指纹连锁块（DFLB）。利用这些连锁块，计算了九个品种在特定染色体区域内的相似性或差异程度。讨论了基于图谱的指纹技术在生物多样性研究和标记辅助选择中的应用。