Mascola J R, Louder M K, Surman S R, Vancott T C, Yu X F, Bradac J, Porter K R, Nelson K E, Girard M, McNeil J G, McCutchan F E, Birx D L, Burke D S
Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, Maryland 20850, USA.
AIDS Res Hum Retroviruses. 1996 Sep 20;12(14):1319-28. doi: 10.1089/aid.1996.12.1319.
Classification of human immunodeficiency virus type 1 (HIV-1) by neutralization serotype may be important for the design of active and passive immunization strategies. Neutralizing antibody serotyping is hindered by the lack of standard reagents and assay format, and by the weak activity of many individual sera. To facilitate cross-clade neutralization analysis, we used an infectivity reduction assay (IRA) and selected clade-specific serum (or plasma) pools from subjects infected with clade B and E HIV-1, respectively. Several serum pools were utilized; some were selected for strong neutralizing activity against intraclade viruses and others were derived from conveniently available samples. Against a panel of 51 clade B and E viruses, serum pools displayed strong neutralization of most intraclade viruses and significantly diminished cross-clade neutralization. Results were confirmed against a blinded panel of 20 viruses. The data indicate that the phylogenetic classification of virus subtypes B and E corresponds to two distinct neutralization serotypes. This approach to neutralizing antibody serotyping may be useful in defining the antigenic relationship among viruses from other clades.
根据中和血清型对1型人类免疫缺陷病毒（HIV-1）进行分类，对于主动和被动免疫策略的设计可能具有重要意义。由于缺乏标准试剂和检测方法，以及许多个体血清的活性较弱，中和抗体血清分型受到了阻碍。为了便于进行跨分支中和分析，我们使用了感染性降低试验（IRA），并分别从感染B和E分支HIV-1的受试者中选择了分支特异性血清（或血浆）池。使用了几个血清池；一些血清池是为了对分支内病毒具有强中和活性而选择的，其他血清池则来自方便获取的样本。针对一组51种B和E分支病毒，血清池对大多数分支内病毒显示出强中和作用，而跨分支中和作用则显著减弱。针对一组20种盲态病毒的结果证实了这一点。数据表明，病毒B和E亚型的系统发育分类对应于两种不同的中和血清型。这种中和抗体血清分型方法可能有助于确定来自其他分支的病毒之间的抗原关系。
