Singh G D, Moxham B J
Department of Dental Surgery and Periodontology, Dundee Dental Hospital and School, University of Dundee, U.K.
Cleft Palate Craniofac J. 1996 Sep;33(5):395-9. doi: 10.1597/1545-1569_1996_033_0395_mcadfd_2.3.co_2.
Biosynthetic activity of the mesenchymal cells within the palatal shelves was determined during cleft palate formation induced by 5-fluoro-2-deoxyuridine (FUDR). The palatal shelves of 30 fetal rat heads with palatal clefts were surveyed at stages corresponding to normal palatogenesis, nucleolar organizer region (NOR) staining being employed to determine cell activity. Comparing cellular activity during normal and cleft palatogenesis, significantly lower counts were recorded for most stages of cleft formation. When anterior or posterior regions were compared, significant changes in NOR counts cell were found at a time corresponding to the pre-elevation stage of normal palatogenesis. At a time equivalent to normal fusion, the shelves in the anterior region showed signs of recovery, but posteriorly significantly lower activity occurred throughout all stages of palate dysmorphogenesis. The depressed level of cellular activity found after treatment with FUDR may be directly or indirectly related to the abolition of an intrinsic shelf-elevation force and, subsequently, cleft palate formation.
在由5-氟-2-脱氧尿苷（FUDR）诱导的腭裂形成过程中，测定了腭突间充质细胞的生物合成活性。对30只患有腭裂的胎鼠头部的腭突在对应于正常腭发生的阶段进行了观察，采用核仁组织区（NOR）染色来确定细胞活性。比较正常和腭裂发生过程中的细胞活性，在腭裂形成的大多数阶段记录到的细胞计数显著降低。当前部或后部区域进行比较时，在对应于正常腭发生的抬高前期，发现NOR计数细胞有显著变化。在相当于正常融合的时间点，前部区域的腭突显示出恢复的迹象，但在整个腭部畸形发生的所有阶段，后部的活性显著降低。用FUDR处理后发现的细胞活性降低水平可能直接或间接与内在的腭突抬高力的消除有关，进而与腭裂的形成有关。
