Singh G D, Moxham B J
Department of Anatomy, School of Medical Sciences, University of Bristol, UK.
J Anat. 1993 Apr;182 ( Pt 2)(Pt 2):163-8.
To provide information concerning biosynthetic activities within the mesenchymal cells of rat palatal shelves, the numbers of nucleolar organiser regions (NORs) were detected using an Ag-NOR staining technique within tissues obtained from coronal sections of 30 fetal heads prepared for light microscopy. The study involved comparisons between stages of palatogenesis and between location along the palatal shelves, anterior and posterior regions being analysed to correspond to the presumptive hard and soft palates. For both anterior and posterior regions, the mean Ag-NOR count/cell was initially high (approximately 3.4 anteriorly and approximately 3.0 posteriorly). The count/cell decreased significantly immediately after elevation but increased again after shelf reorientation and complete fusion. When comparisons were made between anterior and posterior regions of the palatal shelves, no differences were discerned at a stage prior to elevation (day 15) and a stage after the shelves had elevated and completely fused (day 17). Immediately post-elevation (day 16.5), however, the mean count/cell was significantly higher anteriorly. Thus the degree of mesenchymal cell activity, as expressed in terms of protein biosynthesis, can change in the developing palate according to both site and stage of development.
为了提供有关大鼠腭突间充质细胞内生物合成活性的信息，使用银染核仁组织区（Ag-NOR）染色技术，在为光学显微镜检查制备的30个胎头冠状切片的组织中检测核仁组织区（NORs）的数量。该研究涉及腭发育各阶段之间以及腭突不同位置（前部和后部区域分别对应于硬腭和软腭的原基）之间的比较。对于前部和后部区域，每个细胞的平均Ag-NOR计数最初都很高（前部约为3.4，后部约为3.0）。在腭突抬高后，每个细胞的计数显著下降，但在腭突重新定向和完全融合后又再次增加。当对腭突的前部和后部区域进行比较时，在抬高前阶段（第15天）和抬高并完全融合后阶段（第17天）未发现差异。然而，在抬高后即刻（第16.5天），前部每个细胞的平均计数显著更高。因此，就蛋白质生物合成而言，间充质细胞活性的程度在发育中的腭部会根据发育部位和阶段而变化。
