Beck G, Habicht G S
Department of Pathology, SUNY at Stony Brook 11794-8691, USA.
Cytokine. 1996 Jul;8(7):507-12. doi: 10.1006/cyto.1996.0069.
Our previous studies have demonstrated the presence of two of the three vertebrate inflammatory cytokines in invertebrates, namely, interleukin (IL)-1 and tumour necrosis factor (TNF). We studied the coelomic fluid of the echinoderm, Asterias forbesi, to determine whether it contained the third inflammatory cytokine, IL-6. Coelomic fluids were concentrated and then fractionated by a combination of gel sieve chromatography and preparative isoelectric focusing. The M, observed for the invertebrate IL-6-like molecule was approximately 30,000 and the pI was 5.5. Western blot analysis using a polyclonal rabbit antiserum to human IL-6 detected a band at approximately 30,000 Da. The invertebrate IL-6 was inhibited by an antiserum to human IL-6 when used in the B9 assay. Finally, the coelomocytes were found to be capable or releasing the IL-6-like molecule as early as 12 h following stimulation by LPS. These results, together with our other data, show that echinoderms possess correlates of all three vertebrate inflammatory cytokines.
我们之前的研究已证明，三种脊椎动物炎性细胞因子中的两种存在于无脊椎动物中，即白细胞介素（IL）-1和肿瘤坏死因子（TNF）。我们研究了棘皮动物福布斯海星的体腔液，以确定其中是否含有第三种炎性细胞因子IL-6。体腔液经浓缩后，通过凝胶筛层析和制备性等电聚焦相结合的方法进行分级分离。观察到的无脊椎动物IL-6样分子的分子量约为30,000，等电点为5.5。使用针对人IL-6的兔多克隆抗血清进行的蛋白质印迹分析在约30,000 Da处检测到一条带。在B9测定中使用针对人IL-6的抗血清时，无脊椎动物IL-6受到抑制。最后，发现体腔细胞在受到LPS刺激后12小时即可释放IL-6样分子。这些结果与我们的其他数据一起表明，棘皮动物拥有所有三种脊椎动物炎性细胞因子的对应物。
