丙酰-L-肉碱对链脲佐菌素诱导的糖尿病大鼠视网膜电图振荡电位的影响。

Effect of propionyl-L-carnitine on oscillatory potentials in electroretinogram in streptozotocin-diabetic rats.

作者信息

Hotta N, Koh N, Sakakibara F, Nakamura J, Hamada Y, Hara T, Fukasawa H, Kakuta H, Sakamoto N

机构信息

Third Department of Internal Medicine, Nagoya University School of Medicine, Japan.

出版信息

Eur J Pharmacol. 1996 Sep 12;311(2-3):199-206. doi: 10.1016/0014-2999(96)00420-7.

DOI:10.1016/0014-2999(96)00420-7

PMID:8891600

Abstract

The effect of propionyl-L-carnitine, an analogue of L-carnitine, and insulin on the oscillatory potentials of the electroretinogram was determined in rats with streptozotocin-induced diabetes. Propionyl-L-carnitine was administered at a daily dose of 0.5 g/kg by gavage for 4 weeks, while other rats were treated with subcutaneous injections of insulin (8-10 U/day). Both treatments shortened the peak latencies of the oscillatory potentials in the electroretinogram, which were significantly prolonged in untreated diabetic rats (O1, O2 and O3, and sigma (O1 + O2 + O3)) (P < 0.0001 vs. untreated normal rats). A significant decrease in the erythrocyte free carnitine level in diabetic rats was prevented by both treatments. Insulin produced a significant reduction of retinal glucose, sorbitol and fructose levels in diabetic rats, while propionyl-L-carnitine failed to do so. However, both treatments markedly reduced serum lipids levels in the diabetic rats. These findings provide information on the pathogenesis of diabetic retinopathy as well as suggesting the potential therapeutic value of propionyl-L-carnitine for retinopathy.

摘要

在链脲佐菌素诱导的糖尿病大鼠中，测定了左旋肉碱类似物丙酰-L-肉碱和胰岛素对视网膜电图振荡电位的影响。丙酰-L-肉碱以每日0.5 g/kg的剂量灌胃给药4周，而其他大鼠皮下注射胰岛素（8-10 U/天）。两种治疗均缩短了视网膜电图中振荡电位的峰潜伏期，在未治疗的糖尿病大鼠中该潜伏期显著延长（O1、O2和O3以及σ（O1 + O2 + O3））（与未治疗的正常大鼠相比，P < 0.0001）。两种治疗均防止了糖尿病大鼠红细胞游离肉碱水平的显著降低。胰岛素显著降低了糖尿病大鼠的视网膜葡萄糖、山梨醇和果糖水平，而丙酰-L-肉碱未能做到这一点。然而，两种治疗均显著降低了糖尿病大鼠的血脂水平。这些发现提供了有关糖尿病视网膜病变发病机制的信息，并提示了丙酰-L-肉碱对视网膜病变的潜在治疗价值。