Nhieu J T, Nin F, Fleury-Feith J, Chaumette M T, Schaeffer A, Bretagne S
Laboratoire de Parasitologie, Hôpital Henri Mondor, Créteil, France.
Hum Pathol. 1996 Oct;27(10):1107-9. doi: 10.1016/s0046-8177(96)90294-4.
Cyclospora sp is a recently identified coccidia responsible for enteric infection in humans. Most reports have failed to detect this parasite in intestinal biopsies by light microscopy, although the different stages have been ultrastructurally described in jejunum enterocytes. Very recently, some investigators have reported the detection by light microscopy of parasitophorous vacuoles in intestinal biopsies; however, only transmission electron microscopy (TEM) could clearly identify the parasitic stages. To improve the histological diagnosis without using TEM, we have tested different staining methods in biopsies obtained from a patient infected by the human immunodeficiency virus who was shedding Cyclospora oocysts. Hematoxylin stain alone for 15 minutes on 5 micrometer-thick sections of duodenal biopsies was found to be the most efficient method for observing different stages of the parasite. In particular, the banana-shaped merozoites were visualized and appeared very similar to the human coccidia Isopora belli. This simple technique may be useful in diagnosing Cyclospora infection.
环孢子虫是一种最近才被确认的球虫，可导致人类肠道感染。尽管空肠肠细胞中的不同阶段已通过超微结构进行了描述，但大多数报告未能通过光学显微镜在肠道活检组织中检测到这种寄生虫。最近，一些研究人员报告称通过光学显微镜在肠道活检组织中检测到了寄生泡；然而，只有透射电子显微镜（TEM）才能清楚地识别寄生虫的各个阶段。为了在不使用TEM的情况下改善组织学诊断，我们对一名感染了人类免疫缺陷病毒且正在排出环孢子虫卵囊的患者的活检组织进行了不同染色方法的测试。结果发现，在十二指肠活检组织5微米厚的切片上单独使用苏木精染色15分钟是观察该寄生虫不同阶段的最有效方法。特别是，香蕉形裂殖子清晰可见，且看起来与人体球虫贝氏等孢球虫非常相似。这种简单的技术可能有助于诊断环孢子虫感染。
