Asakura Y, Hamajima K, Fukushima J, Mohri H, Okubo T, Okuda K
First Department of Internal Medicine, Yokohama City University School of Medicine, Japan.
Am J Hematol. 1996 Oct;53(2):116-7. doi: 10.1002/(SICI)1096-8652(199610)53:2<116::AID-AJH9>3.0.CO;2-2.
Recently, some individuals who have remained seronegative despite definite exposure to HIV-1 have been reported. Among these individuals, an unusually high frequency of HIV-1 Nef-specific cytotoxic T lymphocytes was observed. Direct injection of plasmid DNA encoding foreign antigen can elicit both cell-mediated immunity and antibody responses (DNA vaccine). We constructed an HIV-1 Nef-expressing plasmid, and we induced HIV-1 Nef-specific cytotoxic T lymphocytes. This is the first report of inducing HIV-1 Nef-specific cytotoxic T lymphocytes by DNA vaccine.
最近，有报道称一些尽管明确接触过HIV-1但血清学仍呈阴性的个体。在这些个体中，观察到HIV-1 Nef特异性细胞毒性T淋巴细胞的频率异常高。直接注射编码外源抗原的质粒DNA可引发细胞介导的免疫和抗体反应（DNA疫苗）。我们构建了一种表达HIV-1 Nef的质粒，并诱导出了HIV-1 Nef特异性细胞毒性T淋巴细胞。这是关于通过DNA疫苗诱导HIV-1 Nef特异性细胞毒性T淋巴细胞的首次报道。
