Siegele D A, Guynn L J
Department of Biology, Texas A&M University, College Station 77843-3258, USA.
J Bacteriol. 1996 Nov;178(21):6352-6. doi: 10.1128/jb.178.21.6352-6356.1996.
Proteins synthesized in Escherichia coli during recovery from starvation were resolved by two-dimensional polyacrylamide gel electrophoresis. Nine outgrowth-specific proteins, which appeared in two kinetic groups, that were not detected in either starved or exponential-phase cells were synthesized. Five other proteins whose rate of synthesis during outgrowth was > or = 5-fold higher than during exponential growth were observed.
通过二维聚丙烯酰胺凝胶电泳对大肠杆菌在从饥饿状态恢复过程中合成的蛋白质进行了分离。发现了九种生长特异性蛋白质,它们出现在两个动力学组中,在饥饿或指数生长期细胞中均未检测到。还观察到另外五种蛋白质,其在生长过程中的合成速率比指数生长期高出5倍或更多。