Isa P, Wood A R, Netherwood T, Ciarlet M, Imagawa H, Snodgrass D R
Moredun Research Institute, Edinburgh, U.K.
Arch Virol. 1996;141(9):1601-12. doi: 10.1007/BF01718285.
DIG-labelled ssRNA probes were prepared from variable regions of VP4 and VP7 cognate genes, and used in hybridization assays for P and G genotyping of group A cell culture-adapted equine rotaviruses and fecal samples collected from foals with and without diarrhea. The probes confirmed known P and G serotypes of sixteen cell culture-adapted strains. From one-hundred and twenty-one rotavirus-positive samples, 83 reacted when tested for their P and G genotype specific probes. From these, 71 were found to contain G3 P12 genotypes, and 11 G14 P12 genotypes. No sample reacted with H1 or L338 P and G genotype probes. This suggests that the equine rotavirus population is conservative, containing predominantly one P genotype and two G genotypes. One isolate (26/94) whose dsRNA was visualized in an agarose gel did not react with any of the equine probes, and was found to belong to G8 and P1 genotypes. This is the fourth example of a single unique equine isolate (after H1, L338, and R-22). The remaining thirty-eight untypable field isolates had no detectable dsRNA after storage for 1 to 3 years.
从VP4和VP7同源基因的可变区制备了地高辛标记的单链RNA探针，并将其用于A组细胞培养适应的马轮状病毒以及从腹泻和未腹泻的小马驹采集的粪便样本的P和G基因分型杂交试验。这些探针证实了16株细胞培养适应株已知的P和G血清型。在121份轮状病毒阳性样本中，83份在检测其P和G基因型特异性探针时发生反应。其中，发现71份含有G3 P12基因型，11份含有G14 P12基因型。没有样本与H1或L338 P和G基因型探针发生反应。这表明马轮状病毒群体具有保守性，主要包含一种P基因型和两种G基因型。一株分离株（26/94），其双链RNA在琼脂糖凝胶中可见，不与任何马探针发生反应，发现属于G8和P1基因型。这是单个独特马分离株的第四个例子（继H1、L338和R-22之后）。其余38份无法分型的现场分离株在储存1至3年后未检测到双链RNA。
