Tabillion R, Kaltwasser H
Arch Microbiol. 1977 May 13;113(1-2):145-51. doi: 10.1007/BF00428595.
Kinetic studies of the uptake of 63Ni were undertaken with two strains of Alcaligenes eutrophus, known to require nickel ions for chemolithotrophic growth. Using carbon dioxide as sole carbon source, growth is stimulated by low concentrations of nickel with optimum concentration for growth stimulation at about 0.3 micron nickel. Higher nickel concentrations were inhibitory. Heterotrophic growth on fructose was not stimulated by nickel ions.--Upon transfer into phosphate buffer freed of heavy metal ions, autotrophically grown cells exhibited rapid uptake of 63Ni which was dependent upon the presence of hydrogen, oxygen and carbon dioxide. Within 60 min nickel was accumulated from the medium, reaching 280-fold concentration in the cells. The observed uptake exhibited a temperature optimum at about 29 degrees C and was markedly inhibited by metabolic inhibitors such as arsenite, iodoacetate, methylene-blue, sodium azide and sodium cyanide. Other heavy metal ions (Zn, Co, Mn and Cu) only slightly inhibited 63Ni-uptake. The efflux of 63Ni from the cells was stimulated by 58NiCl2 and by toluene. These data indicate that nickel ions are accumulated by an energy dependent mechanism in chemolithotrophically grown cells of these strains.
对两株嗜碱产碱杆菌进行了63Ni摄取的动力学研究，已知这两株菌在化能无机营养生长过程中需要镍离子。以二氧化碳作为唯一碳源时，低浓度的镍会刺激生长，刺激生长的最佳镍浓度约为0.3微米镍。更高的镍浓度具有抑制作用。镍离子不会刺激细胞在果糖上的异养生长。——在转移到不含重金属离子的磷酸盐缓冲液中后，自养生长的细胞表现出对63Ni的快速摄取，这取决于氢气、氧气和二氧化碳的存在。在60分钟内，镍从培养基中积累，细胞内浓度达到280倍。观察到的摄取在约29℃时表现出最佳温度，并且受到代谢抑制剂如亚砷酸盐、碘乙酸盐、亚甲蓝、叠氮化钠和氰化钠的显著抑制。其他重金属离子（锌、钴、锰和铜）仅轻微抑制63Ni的摄取。58NiCl2和甲苯会刺激63Ni从细胞中流出。这些数据表明，在这些菌株的化能无机营养生长细胞中，镍离子是通过能量依赖机制积累的。
