Accusport检测马血和血浆中乳酸浓度的准确性。

Accuracy of Accusport for measurement of lactate concentrations in equine blood and plasma.

作者信息

Evans D L, Golland L C

机构信息

Department of Animal Science, University of Sydney, NSW, Australia.

出版信息

Equine Vet J. 1996 Sep;28(5):398-402. doi: 10.1111/j.2042-3306.1996.tb03111.x.

DOI:10.1111/j.2042-3306.1996.tb03111.x

PMID:8894538

Abstract

The aim of this study was to investigate correlations between lactate concentrations in equine whole blood and plasma measured with Accusport1 and Yellow Springs Instruments (YSI2) (2300) methods. The effect of packed cell volume (PCV) on the accuracy of Accusport was also investigated. Blood samples were collected from Thoroughbred horses at 5-10 min intervals after a treadmill exercise test. Blood was added to NaEDTA (for PCV measurement) and to 2 tubes containing lithium heparin anticoagulant (for lactate assays). At concentrations greater than 10 mmol/l, Accusport1 greatly underestimated the blood lactate concentration. Accusport1 also underestimated blood lactate concentration when PCV were greater than 53%. Accusport1 accurately measured plasma lactate concentration in the range 0.8-20 mmol/l.

摘要

本研究的目的是调查使用Accusport1和黄春泉仪器（YSI2）（2300）方法测量的马全血和血浆中乳酸浓度之间的相关性。还研究了血细胞压积（PCV）对Accusport准确性的影响。在跑步机运动试验后，每隔5 - 10分钟从纯种马采集血样。将血液加入NaEDTA（用于测量PCV）和2支含有肝素锂抗凝剂的试管中（用于乳酸测定）。当浓度大于10 mmol/L时，Accusport1大大低估了血乳酸浓度。当PCV大于53%时，Accusport1也低估了血乳酸浓度。Accusport1在0.8 - 20 mmol/L范围内准确测量了血浆乳酸浓度。