Quintero R A, Carreño C A, Yelian F, Evans M I
Department of Obstetrics and Gynecology, Hutzel Hospital, Wayne State University, Detroit, Mich., USA.
Fetal Diagn Ther. 1996 Sep-Oct;11(5):348-56. doi: 10.1159/000264340.
There is a paucity of data regarding healing of fetal membranes. We assessed the repairing ability of a microsurgically-injured monolayer of amnion-derived cells compared to an endothelial cell line.
Amnion-derived cells (FL cells) were grown to confluence in 10% fetal calf serum (FCS). A microsurgical incision was performed under an inverted microscope. The area of each incision was measured at times 0, 6, 12 and 24 h using MCID M4 image analysis software. The experiments were repeated using 1% FCS. The observations were also made replacing the media at 6 and 12 h. Umbilical-cord-derived endothelial cells were used for comparison. The rate of repair of the incision was measured using three methods: (a) absolute closure rate (ACR): (A0-A1)/T, where A0 is the original incisional area, and A1 is the new area at interval T: (b) relative percentage rate (RPR): (A0-A1)/A0/T x 100, and (c) healing rate (HR): (A0-A1)/A0 x 100.
Amnion cells were capable of repairing the microsurgical defect. The ACR decreased over time, and was higher with larger defects. A lower concentration of FCS nor the addition of fresh media altered the repair process significantly. Endothelial cells were significantly faster than amnion cells or amnion-supplemented cells with endothelial media. The RPR remained relatively constant for all groups, suggesting Gompertzian kinetics.
Amnion cells are capable of repairing a surgical defect at a relatively constant percent rate, but are slower than an endothelial cell line. The decreased rate of closure over time may be due to relative contact inhibition. Further studies will concentrate on the molecular and cellular mechanisms involved in this process.
关于胎膜愈合的数据匮乏。我们评估了与内皮细胞系相比，经显微手术损伤的羊膜来源细胞单层的修复能力。
羊膜来源细胞（FL细胞）在含10%胎牛血清（FCS）的培养基中生长至汇合。在倒置显微镜下进行显微手术切口。使用MCID M4图像分析软件在0、6、12和24小时测量每个切口的面积。实验在使用1% FCS的情况下重复进行。在6和12小时更换培养基时也进行了观察。使用脐带来源的内皮细胞作为对照。使用三种方法测量切口的修复率：（a）绝对闭合率（ACR）：(A0 - A1)/T，其中A0是原始切口面积，A1是间隔T时的新面积；（b）相对百分率（RPR）：(A0 - A1)/A0/T×100，以及（c）愈合率（HR）：(A0 - A1)/A0×100。
羊膜细胞能够修复显微手术造成的缺损。ACR随时间下降，且缺损越大ACR越高。较低浓度的FCS或添加新鲜培养基均未显著改变修复过程。内皮细胞比羊膜细胞或用内皮细胞培养基培养的羊膜补充细胞明显更快。所有组的RPR保持相对恒定，提示符合冈珀茨动力学。
羊膜细胞能够以相对恒定的百分比速率修复手术缺损，但比内皮细胞系慢。随着时间推移闭合速率降低可能是由于相对接触抑制。进一步的研究将集中于该过程涉及的分子和细胞机制。
