催产素的生物活性大分子形式。[8-赖氨酸]催产素作为一种合适的配体。
Biologically active macromolecular forms of oxytocin. [8-Lysine]oxytocin as a suitable ligand.
作者信息
Snell C R, Smyth D G
出版信息
Biochem J. 1977 Jul 1;165(1):43-7. doi: 10.1042/bj1650043.
Abstract
[8-Lysine]oxytocin was synthesized on a solid support and possessed an oxytocic activity of 100 +/- 6 units mumol on the isolated rat uterus. The epsilon-carbamoyl, epsilon-3-carboxypropionyl and epsilon-3-carboxybutryl derivatives were prepared and had uterotonic activities of 400, 55 and 50 units/mumol respectively. [8-Lysine]oxytocin was coupled unambiguously through the epsilon-amino group to the carboxyl groups of carboxymethylated dextrans or epsilon-3-carboxypropionly-gelatin. The macromolecular oxytocins were water-soluble and retained signigicant oxytocic activity. [8-Lysine]oxytocin should prove a useful ligand for affinity chromatography of oxytocin-binding proteins.
摘要
[8-赖氨酸]催产素在固相载体上合成，对离体大鼠子宫具有100±6单位/微摩尔的催产活性。制备了ε-氨基甲酰、ε-3-羧基丙酰和ε-3-羧基丁酰衍生物，其子宫收缩活性分别为400、55和50单位/微摩尔。[8-赖氨酸]催产素通过ε-氨基与羧甲基化葡聚糖或ε-3-羧基丙酰明胶的羧基明确偶联。大分子催产素可溶于水，并保留显著的催产活性。[8-赖氨酸]催产素应可成为用于催产素结合蛋白亲和层析的有用配体。
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Biologically active macromolecular forms of oxytocin. [8-Lysine]oxytocin as a suitable ligand.催产素的生物活性大分子形式。[8-赖氨酸]催产素作为一种合适的配体。
Biochem J. 1977 Jul 1;165(1):43-7. doi: 10.1042/bj1650043.
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(1-beta-mercapto-beta,beta-diethylpropionic acid,4-leucine)-8-lysine-vasopressin and (1-beta-mercapto-beta,beta-diethylpropionic acid,4-leucine)oxytocin, compounds possessing antihormonal properties.（1-β-巯基-β，β-二乙基丙酸，4-亮氨酸）-8-赖氨酸-加压素和（1-β-巯基-β，β-二乙基丙酸，4-亮氨酸）催产素，具有抗激素特性的化合物。
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Oxytocin and lysine-vasopressin with N5,N5-dialkylglutamine in the 4 position: effect of introducing sterically hindered groups into the hydrophilic cluster of neurohypophyseal hormones.4位带有N5,N5 - 二烷基谷氨酰胺的催产素和赖氨酸加压素：将空间位阻基团引入神经垂体激素亲水簇的效果
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