罗汉果提取物的抗氧化特性。

Antioxidant property of Fructus Momordicae extract.

作者信息

Shi H, Hiramatsu M, Komatsu M, Kayama T

机构信息

Department of Neurosurgery, Yamagata University School of Medicine, Japan.

出版信息

Biochem Mol Biol Int. 1996 Dec;40(6):1111-21. doi: 10.1080/15216549600201753.

DOI:10.1080/15216549600201753

PMID:8988323

Abstract

The antioxidant effect of Fructus Momordicae extract, FME (mogrosides 75 approximately 80%), was studied. FME reduced the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and scavenged superoxide radicals (O2-) generated by a hypoxanthine and xanthine oxidase system. It also scavenged hydroxyl radicals (.OH) generated by Fenton reaction. In addition, FME inhibited Fe(II) induced lipid peroxidation in rat cortex homogenates in a dose-dependent manner, as indicated by decreased thiobarbituric acid-reactive substances (TBARS) formation. Oral administration of FME inhibited TBARS and malonaldehyde (MDA) formation in the ipsilateral cortex 30 min after iron-salt injection into the left cortex of rat. FME showed inhibitory effect on 4-hydroxy-2(E)-nonenal (4-HNE) formation induced by Fe(III) injection into the rat cortex. These data suggest that Fructus Momordicae extract has an antioxidant activity against free radicals and lipid peroxidation.

摘要

对罗汉果提取物（FME，罗汉果甜苷含量约为75%至80%）的抗氧化作用进行了研究。FME可还原稳定自由基1,1-二苯基-2-苦基肼（DPPH），并清除次黄嘌呤和黄嘌呤氧化酶系统产生的超氧阴离子自由基（O2-）。它还能清除芬顿反应产生的羟基自由基（·OH）。此外，FME以剂量依赖的方式抑制铁离子（Fe(II)）诱导的大鼠皮质匀浆脂质过氧化，表现为硫代巴比妥酸反应性物质（TBARS）生成减少。给大鼠左侧皮质注射铁盐30分钟后，口服FME可抑制同侧皮质中TBARS和丙二醛（MDA）的生成。FME对向大鼠皮质注射铁离子（Fe(III)）诱导的4-羟基-2（E）-壬烯醛（4-HNE）生成具有抑制作用。这些数据表明，罗汉果提取物对自由基和脂质过氧化具有抗氧化活性。