Yamamoto M, Sobue G, Yamamoto K, Terao S, Mitsuma T
Department of Neurology, Nagoya University School of Medicine, Japan.
Neurochem Res. 1996 Aug;21(8):929-38. doi: 10.1007/BF02532343.
The steady-state mRNA levels of the four neurotrophic factors of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and glial cell line-derived neurotrophic factor (GDNF) and their receptors (p75NGFR, trkA, trkB and trkC) in the adult human peripheral nervous system (PNS) as well as nonneural tissues were examined using quantitative reverse transcription-polymerase chain reaction (RT-PCR). NGF and BDNF mRNA levels were high in the heart and spleen as well as in the dorsal root ganglia (DRG) and spinal cord, showing similar spatial expression patterns, while NT-3 mRNA levels were more pronounced in the liver and spleen. In contrast to these neurotrophins, GDNF mRNA expression occurred at the highest levels in the muscle, and it was also comparatively high in the spinal cord. p75NGFR mRNA was expressed extensively throughout the PNS tissues and in the spleen. The spatial expression patterns differed among trkA, and trkB and trkC mRNAs. trkA mRNA was greatly expressed in the DRG, sympathetic ganglia and spleen, while the trkB and trkC mRNA levels were high in the DRG, spinal cord and brain. The levels of trkB and trkC mRNAs with tyrosine kinase domain, compared to those with extracellular domain, were relatively high in the DRG, whereas they were low in the spinal cord and brain. The spatial patterns of the distributions of neurotrophic factors and their receptors mRNA levels in the adult human PNS and nonneural tissues are largely similar to those reported in other mammals, but these findings provide further, more specific, understanding relevant to the therapeutic approach to human diseases.
采用定量逆转录-聚合酶链反应（RT-PCR）检测了成年人类外周神经系统（PNS）以及非神经组织中神经生长因子（NGF）、脑源性神经营养因子（BDNF）、神经营养素-3（NT-3）和胶质细胞系源性神经营养因子（GDNF）这四种神经营养因子及其受体（p75NGFR、trkA、trkB和trkC）的稳态mRNA水平。NGF和BDNF的mRNA水平在心脏、脾脏以及背根神经节（DRG）和脊髓中较高，呈现出相似的空间表达模式，而NT-3的mRNA水平在肝脏和脾脏中更为明显。与这些神经营养因子不同，GDNF的mRNA表达在肌肉中水平最高，在脊髓中也相对较高。p75NGFR的mRNA在整个PNS组织和脾脏中广泛表达。trkA、trkB和trkC的mRNA的空间表达模式有所不同。trkA的mRNA在DRG、交感神经节和脾脏中大量表达，而trkB和trkC的mRNA水平在DRG、脊髓和脑中较高。与具有细胞外结构域的trkB和trkC的mRNA水平相比，具有酪氨酸激酶结构域的trkB和trkC的mRNA水平在DRG中相对较高，而在脊髓和脑中较低。成年人类PNS和非神经组织中神经营养因子及其受体mRNA水平的分布空间模式在很大程度上与其他哺乳动物报道的相似，但这些发现为人类疾病的治疗方法提供了更深入、更具体的认识。
