Müller N, Zimmermann V, Hentrich B, Gottstein B
Institute of Parasitology, University of Berne, Switzerland.
J Clin Microbiol. 1996 Nov;34(11):2850-2. doi: 10.1128/jcm.34.11.2850-2852.1996.
A recently described PCR test for the identification of Neospora caninum and Toxoplasma gondii has been further developed and optimized in view of its practicability for routine diagnostic application. The N. caninum-specific PCR was adapted to the diagnostic operating standard of the T. gondii-specific PCR in that the uracil DNA glycosidase system was introduced, which eliminates potential carry-over contaminations of amplified target DNA from previous reactions. Furthermore, both PCR tests were optimized by including a DNA hybridization immunoassay based on the use of the commercially available Gen-eti-k DEIA kit. This assay allowed highly sensitive and specific detection of respective DNA amplification products and thus substantially facilitated the reading and interpretation of the test results.
最近描述的一种用于鉴定犬新孢子虫和刚地弓形虫的聚合酶链反应（PCR）检测方法，鉴于其在常规诊断应用中的实用性，已得到进一步开发和优化。犬新孢子虫特异性PCR采用了与刚地弓形虫特异性PCR相同的诊断操作标准，即引入尿嘧啶DNA糖基化酶系统，以消除先前反应中扩增靶DNA的潜在残留污染。此外，两种PCR检测方法均通过纳入基于使用市售Gen-eti-k DEIA试剂盒的DNA杂交免疫分析进行了优化。该分析能够对各自的DNA扩增产物进行高度灵敏和特异的检测，从而极大地便于检测结果的读取和解释。
