Bretagne S, Costa J M, Vidaud M, Tran J, Nhieu V, Fleury-Feith J
Laboratoire de Parasitologie, Hôpital Henri Mondor, Créteil, France.
J Infect Dis. 1993 Dec;168(6):1585-8. doi: 10.1093/infdis/168.6.1585.
The prevalence of pulmonary toxoplasmosis was assessed by a prospective analysis of 144 bronchoalveolar lavage (BAL) samples using competitive polymerase chain reaction (PCR) to avoid false-negative results due to PCR techniques. Six samples were excluded because they contained amplification reaction inhibitors. None of the samples from the 37 immunocompetent patients and only 1 sample (1.7%) from the 59 immunocompromised patients without human immunodeficiency virus infection were PCR-positive. In contrast, Toxoplasma gondii DNA was found in 6 (14%) of 42 samples from patients with AIDS. All 6 patients had < or = 40 CD4 cells/microL and anti-Toxoplasma antibodies, and 5 had other sites of Toxoplasma infection. Six other AIDS patients who had received treatment for cerebral toxoplasmosis were PCR-negative. Thus, pulmonary toxoplasmosis is frequent in AIDS patients who have other sites of Toxoplasma infection and low CD4 lymphocyte counts and who are not receiving prophylaxis.
通过对144份支气管肺泡灌洗（BAL）样本进行前瞻性分析，采用竞争性聚合酶链反应（PCR）评估肺弓形虫病的患病率，以避免因PCR技术导致的假阴性结果。6份样本因含有扩增反应抑制剂而被排除。37名免疫功能正常患者的样本均未呈PCR阳性，59名无人类免疫缺陷病毒感染的免疫功能低下患者中只有1份样本（1.7%）呈PCR阳性。相比之下，42份艾滋病患者的样本中有6份（14%）检测到弓形虫DNA。所有6名患者的CD4细胞计数均≤40/μL且有抗弓形虫抗体，其中5名患者有其他部位的弓形虫感染。另外6名接受过脑弓形虫病治疗的艾滋病患者PCR检测为阴性。因此，肺弓形虫病在有其他部位弓形虫感染、CD4淋巴细胞计数低且未接受预防治疗的艾滋病患者中很常见。
