Král'ová I
Institute of Parasitology, Slovak Academy of Sciences, Kosice, Slovak Republic.
Parasitol Res. 1996;82(8):668-71. doi: 10.1007/s004360050183.
Two morphologically similar fish tapeworms, Proteocephalus exiguus and P. percae, were differentiated by the random amplified polymorphic DNA (RAPD) marker and Southern-blot hybridization techniques. Four geographic isolates of P. exiguus and two of P. percae were studied using eight arbitrary decamer oligonucleotides as primers. Species-specific RAPD fragments enabled reliable separation of the taxa studied. Generally, 1-3 fragments specific for P. percae and 1-4 P. exiguus-specific fragments were detected using individual primers. Intraspecific differences in P. percae depended on the primer used. The profiles generated by the primers OPA 04 and OPA 12 showed an intraspecific variation, whereas six other primers tested revealed indistinguishable banding patterns. On the other hand, intraspecific variability of P. exiguus, detected among four geographic isolates, was evident using all primers. Additionally, the utilization of two species-specific RAPD markers as effective probes was demonstrated. Distinct differences between P. exiguus and P. percae were revealed using each of the restriction enzyme/probe combinations.
通过随机扩增多态性DNA（RAPD）标记和Southern杂交技术区分了两种形态相似的鱼类绦虫，即微小前殖吸虫（Proteocephalus exiguus）和鲈前殖吸虫（P. percae）。以8种任意的十聚体寡核苷酸为引物，研究了微小前殖吸虫的4个地理分离株和鲈前殖吸虫的2个地理分离株。物种特异性RAPD片段能够可靠地分离所研究的分类单元。一般来说，使用单个引物可检测到1 - 3个鲈前殖吸虫特异性片段和1 - 4个微小前殖吸虫特异性片段。鲈前殖吸虫的种内差异取决于所使用的引物。引物OPA 04和OPA 12产生的图谱显示有种内变异，而测试的其他六种引物显示出难以区分的条带模式。另一方面，在四个地理分离株中检测到的微小前殖吸虫种内变异性，使用所有引物均很明显。此外，还证明了两种物种特异性RAPD标记作为有效探针的用途。使用每种限制性内切酶/探针组合均揭示了微小前殖吸虫和鲈前殖吸虫之间的明显差异。
