Jobet E, Bougnoux M E, Morand S, Rivault C, Cloarec A, Hugot J P
Laboratoire de Parasitologie-Mycologie, Hôpital Ambroise-Paré, Boulogne.
Parasite. 1998 Mar;5(1):47-50. doi: 10.1051/parasite/1998051047.
Random amplified DNA markers (RAPD; Williams et al., 1990) were used to obtained specific RAPD fragments characterising different species of oxyuroids. We tested six species of worms parasitizing vertebrates or invertebrates: Passalurus ambiguus Rudolphi, 1819, parasite of Leporids; Syphacia obvelata (Rudolphi, 1802) Seurat, 1916, a parasite of rodents; Blatticola blattae (Graeffe, 1860) Chitwood, 1932 parasite of the cockroach Blattella germanica; Hammerschmidtiella diesingi (Hammerschmidt, 1838) Chitwood, 1932 and Thelastoma bulhoesi (Magalhaes, 1990) Travassos, 1929, parasites of the cockroach Periplaneta americana, and an undescribed parasite species of a passalid insect from New Caledonia. Among 15 oligonucleotides tested, nine produced several specific bands allowing the interspecific discrimination.
随机扩增多态性DNA标记（RAPD；Williams等人，1990）被用于获得表征不同类尖尾线虫物种的特异性RAPD片段。我们测试了六种寄生于脊椎动物或无脊椎动物的蠕虫：鲁道夫钝尾线虫（Passalurus ambiguus Rudolphi，1819），兔科动物的寄生虫；奥氏管尾线虫（Syphacia obvelata (Rudolphi, 1802) Seurat，1916），啮齿动物的寄生虫；德国小蠊管氏线虫（Blatticola blattae (Graeffe, 1860) Chitwood，1932），德国小蠊的寄生虫；迪氏哈氏线虫（Hammerschmidtiella diesingi (Hammerschmidt, 1838) Chitwood，1932）和布氏泰氏线虫（Thelastoma bulhoesi (Magalhaes, 1990) Travassos，1929），美洲大蠊的寄生虫，以及来自新喀里多尼亚的一种扁甲昆虫的未描述寄生虫物种。在测试的15种寡核苷酸中，9种产生了几条特异性条带，可用于种间鉴别。
