心脏细胞中的脂氧合酶活性。

Lipoxygenase activity in heart cells.

作者信息

Breitbart E, Sofer Y, Shainberg A, Grossman S

机构信息

Department of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.

出版信息

FEBS Lett. 1996 Oct 21;395(2-3):148-52. doi: 10.1016/0014-5793(96)01017-4.

DOI:10.1016/0014-5793(96)01017-4

PMID:8898083

Abstract

Arachidonic acid (AA) metabolism via the lipoxygenase (LOX) pathway in rat hearts and in cultured rat cardiomyocytes was investigated using 1-[14C]AA. LOX activity was detected in the microsomal fraction, in the high speed supernatant prepared from rat hearts and in rat cardiomyocyte supernatant. LOX products from all fractions comigrated in thin layer chromatography as 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE. Enzyme linked immunosorbent assay for 12-HETE showed its formation by the microsomal fraction, the ammonium sulfate (AS) pellet, and by rat cardiomyocyte supernatant, while radioimmunoassay for 15-HETE showed its formation only by the AS pellet. The properties of LOX in each fraction are reported here.

摘要

利用1-[14C]花生四烯酸，研究了花生四烯酸（AA）在大鼠心脏和培养的大鼠心肌细胞中通过脂氧合酶（LOX）途径的代谢。在微粒体部分、从大鼠心脏制备的高速上清液以及大鼠心肌细胞上清液中检测到了LOX活性。所有部分的LOX产物在薄层色谱中作为12-羟基二十碳四烯酸（12-HETE）和15-HETE一起迁移。针对12-HETE的酶联免疫吸附测定表明，微粒体部分、硫酸铵（AS）沉淀以及大鼠心肌细胞上清液均可形成12-HETE，而针对15-HETE的放射免疫测定表明，只有AS沉淀可形成15-HETE。本文报道了各部分中LOX的性质。