Dvorkin V M, Kir'ianov G I, Vaniushin B F
Biokhimiia. 1977 Jun;42(6):1001-5.
DNA associated with nuclear envelopes was isolated from rat liver nuclear lysate using centrifugation in sucrose gradient. The specific radioactivity of DNA obtained in nuclear envelope was compared with that of overall nuclear DNA at different stages of the S-period after partial hepatectomy and 1-15 min following intravenous injection of 3H-thymidine. It was found that at various steps of the cell cycle (19, 24 and 27 hrs following partial hepatectomy) the specific radioactivities of nuclear membrane-linked and overall nuclear DNAs do not show any significant differences. No noticeable selective synthesis or initiation of DNA replication on the nuelear envelope were observed either.
使用蔗糖梯度离心法从大鼠肝细胞核裂解物中分离出与核膜相关的DNA。在部分肝切除术后S期的不同阶段以及静脉注射3H-胸腺嘧啶核苷后1 - 15分钟，将核膜中获得的DNA的比放射性与整个核DNA的比放射性进行比较。结果发现，在细胞周期的各个阶段（部分肝切除术后19、24和27小时），核膜相关DNA和整个核DNA的比放射性没有显示出任何显著差异。在核膜上也未观察到明显的DNA选择性合成或DNA复制起始。
