Lemke R, Gadient R A, Schliebs R, Bigl V, Patterson P H
Paul Flechsig Institute for Brain Research, University of Leipzig, Medical Faculty, Germany.
Neurosci Lett. 1996 Sep 13;215(3):205-8. doi: 10.1016/0304-3940(96)12986-4.
Very little is known about the action of the leukemia inhibitory factor (LIF) in the central nervous system. To analyze LIF expression in adult rat brain, we used non-radioactive in situ hybridization with digoxigenin-labeled RNA probes, in combination with immunohistochemistry to identify the cell types expressing LIF mRNA. Hippocampal pyramidal cells, hilar interneurons and granule cells of the dentate gyrus display strong hybridization signals, while neurons of the cerebral cortex, cerebellum, cholinergic basal forebrain nuclei, and the striatum are labeled as well. The most prominent expression in cerebral cortex is in layers IV and VI. Glial fibrillary acidic protein-positive astrocytes did not display a detectable LIF hybridization signal suggesting that LIF mRNA is predominantly expressed in neurons in the normal, adult rat brain, although a presence in resting microglial cells cannot be excluded.
关于白血病抑制因子（LIF）在中枢神经系统中的作用，我们所知甚少。为了分析成年大鼠脑中LIF的表达情况，我们使用了地高辛配体标记的RNA探针进行非放射性原位杂交，并结合免疫组织化学来鉴定表达LIF mRNA的细胞类型。海马锥体细胞、齿状回的门区中间神经元和颗粒细胞呈现出强烈的杂交信号，而大脑皮层、小脑、胆碱能基底前脑核和纹状体的神经元也被标记。大脑皮层中最显著的表达位于IV层和VI层。胶质纤维酸性蛋白阳性的星形胶质细胞未显示出可检测到的LIF杂交信号，这表明在正常成年大鼠脑中，LIF mRNA主要在神经元中表达，尽管不能排除其在静息小胶质细胞中的存在。
