Lorenz E, Stauffer G V
Department of Microbiology, University of Iowa, Iowa City 52242, USA.
FEMS Microbiol Lett. 1996 Nov 1;144(2-3):229-33. doi: 10.1111/j.1574-6968.1996.tb08535.x.
Inactivation of either of the two MetR binding sites centered at bp -143 and 121 relative to the +1 transcription start site results in reduced glyA-lacZ expression in a wild-type strain below the level seen in a metR mutant. This reduced expression is dependent on the side of the DNA helix MetR binds relative to the RNA polymerase binding site. Thus, a single MetR dimer bound to the DNA may play a physiological role in maintaining appropriate glyA gene expression, functioning as a repressor under low MetR conditions.
