一种可靠的高尔基-科普施改良法。

A reliable Golgi-Kopsch modification.

作者信息

Riley J N

出版信息

Brain Res Bull. 1979 Jan-Feb;4(1):127-9. doi: 10.1016/0361-9230(79)90067-4.

Abstract

A reliable Golgi-Kopsch variant is described. Tissue is fixed with 1% paraformaldehyde, 1% glutaraldehyde in 0.1 M phosphate buffer, pH 7.2. Tissue slabs (2.5--4 thick) are immersed for 5--7 days in a solution containing 3.5 g potassium dichromate, 15 g sucrose, 1.65 ml formaldehyde in 100 ml distilled water, washed briefly in 0.75% silver nitrate, and immersed in 0.75% silver nitrate for 2--3 days. Procedures for cutting, counterstaining, and handling sections are described.

摘要

描述了一种可靠的高尔基-科普施变体。组织用0.1M pH 7.2的磷酸盐缓冲液中的1%多聚甲醛、1%戊二醛固定。将组织块（2.5 - 4毫米厚）浸入含有3.5克重铬酸钾、15克蔗糖、1.65毫升甲醛的100毫升蒸馏水溶液中5 - 7天，在0.75%硝酸银中短暂冲洗，然后浸入0.75%硝酸银中2 - 3天。描述了切片、复染和处理切片的步骤。