Ozawa R, Matsumoto S
Insect Toxicology and Physiology, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Insect Biochem Mol Biol. 1996 Mar;26(3):259-65. doi: 10.1016/0965-1748(95)00088-7.
In the silkworm, Bombyx mori, production of the sex pheromone bombykol is regulated by a neurohormone termed PBAN. We have detected the activity of acyl CoA reductase in the pheromone gland of B. mori by using palmitoyl CoA as a substrate. The acyl CoA reductase requires NADPH, but not NADH, as a proton dono. When the pheromone gland was incubated with the PBAN fragment peptide TKYFSPRLamide, palmitoyl CoA was incorporated and converted into the corresponding C16 alcohols. Radio HPLC analysis revealed that these C16 alcohols were hexadecan-1-ol (81.2%), (Z)-11-hexadecen-1-ol (12.3%), and (E, Z)-10, 12-hexadecadien-1-ol (= bombykol, 6.5%). The production of C16 alcohols in the pheromone gland was inhibited by the known bombykol biosynthesis inhibitors EDTA, LaCl3, W-7, trifluoperazine, p-nitrophenyl phosphate, NaF and compactin. By contrast, when the pheromone gland homogenate was incubated in the presence of palmitoyl CoA and NADPH, production of C16 alcohols was affected by compactin, W-7 and trifluoperazine, but not by EDTA, LaCl3, p-nitrophenyl phosphate and NaF. These results indicate that compactin, W-7 and trifluoperazine directly suppress the step catalyzed by acyl CoA reductase, whereas EDTA, LaCl3, pNPP, and NaF inhibit bombykol production by affecting other biochemical steps in the signal transduction of PBAN action. The present results also imply that PBAN regulates the step catalyzed by acyl CoA reductase and that palmitoyl CoA could be used as a substrate of the acyl CoA reductase that regulates bombykol biosynthesis.
在家蚕（Bombyx mori）中，性信息素蚕蛾醇的产生受一种名为PBAN的神经激素调节。我们以棕榈酰辅酶A为底物，检测了家蚕性信息素腺体中酰基辅酶A还原酶的活性。该酰基辅酶A还原酶需要NADPH而非NADH作为质子供体。当性信息素腺体与PBAN片段肽TKYFSPRLamide一起孵育时，棕榈酰辅酶A被掺入并转化为相应的C16醇。放射性高效液相色谱分析表明，这些C16醇为十六烷 - 1 - 醇（81.2%）、（Z） - 11 - 十六碳烯 - 1 - 醇（12.3%）和（E，Z） - 10，12 - 十六碳二烯 - 1 - 醇（=蚕蛾醇，6.5%）。性信息素腺体中C16醇的产生受到已知的蚕蛾醇生物合成抑制剂EDTA、LaCl3、W - 7、三氟拉嗪、对硝基苯磷酸酯、NaF和洛伐他汀的抑制。相比之下，当性信息素腺体匀浆在棕榈酰辅酶A和NADPH存在下孵育时，C16醇的产生受洛伐他汀、W - 7和三氟拉嗪影响，但不受EDTA、LaCl3、对硝基苯磷酸酯和NaF影响。这些结果表明，洛伐他汀、W - 7和三氟拉嗪直接抑制酰基辅酶A还原酶催化的步骤，而EDTA、LaCl3、对硝基苯磷酸酯和NaF通过影响PBAN作用信号转导中的其他生化步骤来抑制蚕蛾醇的产生。目前的结果还表明，PBAN调节酰基辅酶A还原酶催化的步骤，并且棕榈酰辅酶A可作为调节蚕蛾醇生物合成的酰基辅酶A还原酶的底物。
